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| 2. |  | GREEN, M.; LIMA, W. A. A.; FIGUEIREDO, A. F. de; ATROCH, A. L.; LOPES, R.; CUNHA, R. N. V. da; TEIXEIRA, P. C. Heat-treatment and germination of oil palm seeds (Elaeis guineensis Jacq.). Journal of Seed Science, v. 35, n. 3, p. 296-301, 2013.| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Solos. |
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| 3. | | COSTA, L. V.; LOPES, R.; LOPES, M. T. G.; FIGUEIREDO, A. F. de; BARROS, W. S. Cross compatibility of domesticated hot pepper and cultivated sweet pepper. Crop Breeding and Applied Biotechnology, Londrina, v. 9, n. 1, p. 37-44, Mar. 2009.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 4. | | COSTA, L. V.; LOPES, R.; LOPES, M. T. G.; FIGUEIREDO, A. F. de; BARROS, W. S.; ALVES, S. R. M. Cross compatibility of domesticated hot pepper and cultivated sweet pepper. Crop Breeding and Applied Biotechnology, v. 9, n. 1, p. 37-44, Mar. 2009.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 5. | | COSTA, L. V.; LOPES, R.; LOPES, M. T. G.; FIGUEIREDO, A. F. de; BARROS, W. W.; ALVES, S. R. M. Cross compatibility of domesticated hot pepper and cultivated sweet pepper. Crop Breeding and Applied Biotechnology, Londrina, v. 9, n. 1, p. 37-44, Mar. 2009.| Biblioteca(s): Embrapa Hortaliças. |
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| 8. | | SILVA, H. C. da; FIGUEIREDO, A. F. de; TUCCI, C. A. F.; SILVA, J. F. da. Qualidade das sementes de caupi (Vigna unguiculata (L.) Walp.) comercializadas em Manaus, estado do Amazonas. Revista da Universidade do Amazonas. Serie: Ciencias Agrarias, Manaus, v. 8, n.1/2, p. 33-40, jan./dez. 1999.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 10. | | HARA, F. A. dos S.; TUCCI, C. A. F.; FIGUEIREDO, A. F. de; PEREIRA, J. O. Avaliação de genótipos de milho (Zea mays L.) na implantação de um sistema agroflorestal de cupuaçu (Theobroma grandiflorum (Willd, ex Spreng) Schum) e pupunha (Bactris gasipae HBK) no estado do Amazonas Revista da Universidade do Amazonas. Serie: Ciencias Agrarias, Manaus, v.7, n.1/2, p.1-18, jan./dez. 1998.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 22 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/02/2009 |
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Data da última atualização: |
10/02/2009 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
MARTINS, P. K.; JORDÃO, B. Q.; YAMANAKA, N.; FARIAS, J. R. B.; BENEVENTI, M. A.; BINNECK, E.; FUGANTI, R.; STOLF, R.; NEPOMUCENO, A. L. |
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Afiliação: |
Polyana K. Martins, UEL; Berenice Q. Jordão, UEL; Naoki Yamanaka, JIRCAS; José Renato Bouças Farias. CNPSo; Magda Aparecida Beneventi, UEL; Eliseu Binneck, CNPSo; Renata Fuganti, UEL; Renata Stolf, UEL; Alexandre Lima Nepomuceno, CNPSo. |
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Título: |
Differential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 31, n. 2, p. 512-521, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Drought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water- stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the Arabidopsis thaliana phosphatidylinositol transfer protein PITP and the auxin-independent growth regulator 1 (axi 1). The hydroponic experiments showed that after 100 min outside the nutrient solution photosynthesis completely stopped, stomata closed and leaf temperature rose. Both stress induction treatments produced significant decrease in the mitotic indices of root cells. Axi 1, PITP and bHLH were not only differentially expressed during dehydration in the hydroponics experiments but also during induced drought in the pot experiments. Although, there were differences between the two sets of experiments in the time at which up or down regulation occurred, the expression pattern of all three transcripts was related. Similar gene expression and cytological analysis results occurred in both systems, suggesting that hydroponics could be used to simulate drought detection by roots growing in soil and thus facilitate rapid and easy root sampling. MenosDrought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water- stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the Arabidopsis thaliana phosphatidylinositol transfer protein PITP and the auxin-independent growth regulator 1 (axi 1). The hydroponic experiments showed that after 100 min outside the nutrient solution photosynthesis completely stopped, stomata closed and leaf temperature rose. Both stress induction treatments produced significant decrease in the mitotic indices of root cells. Axi 1, PITP and bHLH were not only differentially expressed during dehydration in the hydroponics experiments but also during induced drought in the pot experiments. Although, there were differences between the two sets of experiments in the time at which up or down regulation occurred, the expression pattern of all three transcripts was related. Simila... Mostrar Tudo |
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Categoria do assunto: |
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URL: |
https://www.scielo.br/pdf/gmb/v31n2/a19v31n2.pdf
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Marc: |
LEADER 02469naa a2200217 a 4500
001 1471496
005 2009-02-10
008 2008 bl --- 0-- u #d
100 1 $aMARTINS, P. K.
245 $aDifferential gene expression and mitotic cell analysis of the drought tolerant soybean (Glycine max L. Merrill Fabales, Fabaceae) cultivar MG/BR46 (Conquista) under two water deficit induction systems.
260 $c2008
520 $aDrought cause serious yield losses in soybean (Glycine max), roots being the first plant organ to detect the water- stress signals triggering defense mechanisms. We used two drought induction systems to identify genes differentially expressed in the roots of the drought-tolerant soybean cultivar MG/BR46 (Conquista) and characterize their expression levels during water deficit. Soybean plants grown in nutrient solution hydroponically and in sand-pots were submitted to water stress and gene expression analysis was conducted using the differential display (DD) and real time polymerase chain reaction (PCR) techniques. Three differentially expressed mRNA transcripts showed homology to the Antirrhinum majus basic helix-loop-helix transcription factor bHLH, the Arabidopsis thaliana phosphatidylinositol transfer protein PITP and the auxin-independent growth regulator 1 (axi 1). The hydroponic experiments showed that after 100 min outside the nutrient solution photosynthesis completely stopped, stomata closed and leaf temperature rose. Both stress induction treatments produced significant decrease in the mitotic indices of root cells. Axi 1, PITP and bHLH were not only differentially expressed during dehydration in the hydroponics experiments but also during induced drought in the pot experiments. Although, there were differences between the two sets of experiments in the time at which up or down regulation occurred, the expression pattern of all three transcripts was related. Similar gene expression and cytological analysis results occurred in both systems, suggesting that hydroponics could be used to simulate drought detection by roots growing in soil and thus facilitate rapid and easy root sampling.
700 1 $aJORDÃO, B. Q.
700 1 $aYAMANAKA, N.
700 1 $aFARIAS, J. R. B.
700 1 $aBENEVENTI, M. A.
700 1 $aBINNECK, E.
700 1 $aFUGANTI, R.
700 1 $aSTOLF, R.
700 1 $aNEPOMUCENO, A. L.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 31, n. 2, p. 512-521, 2008.
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